IMSC 2020

Short Courses

Find below information on each of IMSC2020's Short Courses. This page is regularly updated.

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Short Courses Description

Short course outline & topics

Target Audience: It will it be mainly for beginners and also for advanced students.

Description

A two-day course on fundamentals of mass spectrometry aimed to give principles and applications of MS of organic and bioorganic molecules.

The course starts with an overview of modern mass spectrometry and a description of the main ionization techniques, for volatile and polar molecules, and ambient MS techniques, followed by an overview on in-space and in- time analyzers.

The course develops presenting isotopic patterns, ion formation and ion internal energy, together with some criteria for interpretation of mass spectra.

High-resolution MS and accurate mass measurements for molecular formula determination and different techniques of tandem mass spectrometry complete the course.

For each topic, exercises involving active participation by students and applications in different fields will be presented.

Summary

·         Mass spectrometry: an overview

·         Ionization techniques: volatile molecules: EI, CI

·         Ionization techniques: polar molecules : ESI, APCI, APPI, MALDI

·         Ambient MS

·         Analyzers: BE, Q, Tof, Ion trap, Orbitrap, FTICR

·         High resolution mass spectrometry and accurate mass measurements

·         Molecular formula determination

·         Tandem mass spectrometry

·         Principles of interpretation of MS and MS/MS spectra

·         Exercises and applications

 

Methodology

Lectures

 

Language

English

Tutor

Dr. Gianluca Giorgi 

1990: Ph.D. in Chemistry. 

1997-2003: Director of "Interdepartmental Center of Analysis and Structural Determinations" of the University of Siena. 

2000-2008: Researcher of organic chemistry at the Faculty of Mathematics, Physics and Natural Sciences of the University of Siena.

2008-: Associate Professor of Organic Chemistry at the Department of Biotechnology, Chemistry and Pharmacy of the University of Siena.

2017: National Academic Qualification as Full Professor in Organic Chemistry.

2008-2010, 2014-2016: President of the Division of Mass Spectrometry of the Italian Chemical Society.

2014-2018: Representative of Area A (Europe and Africa) in the International Mass Spectrometry Foundation.

2018: Chair of the 22nd International Mass Spectrometry Conference held in Florence (Italy) on August 26-31, 2018.

His scientific research concerns the study of gas phase ion chemistry of organic and bioorganic compounds using different approaches of mass spectrometry and computational methods, with special regards to structural, regio- and stereochemical features and aggregation properties.

He is author and co-author of 190 publications in international journals, monographs, editorials and more than 250 communications at national and international conferences.

Hirsh h-index: 27 Total number of citations: 2564, Total number of citations without self-citation: 2361.

He is member of the Editorial Board of the Journal of Mass Spectrometry and member of the Society Advisory Board of the Portal "Chemistry", both published by Wiley.

He is member of the American Society of Mass Spectrometry and of the Italian Chemical Society.

Short course outline & topics

Target Audience: Beginners on high resolution mass spectrometry

 

Fundamental Concepts for HRMS

• Mass Terminology: nominal, average, accurate, exact, monoisotopic

• Mass Separation : resolution, resolving power

• Mass Measurement: centroid, profile, peak top, millimass units vs. parts per million

• Mass Defect: the story in the decimal places

• Isotopes

 

Instrument Types and Concepts

• Major operating principles and challenges in

• Time of Flight MS: delayed extraction, reflectron, orthogonal acceleration

• Fourier Transform Ion Cyclotron Resonance (FTICR) MS: Magnet, cell types, ressure/vacuum requirements, ultrahigh resolution

• Orbitrap MS: ion storage and injection techniques, MS/MS options, scan speed vs.resolution

• Quadrupole and Quadrupole--based ion traps: resolution capability, impact on hybrid S/MS systems

 

MS/MS

• Major options

– Resolution in each mass analyzer

– Scan speed compatibility ( full scan and MS/MS switching, parallel processing options)

• Fragmentation Options: CAD, ETD, etc.

 

Qualitative Analysis

• HRMS based options for metabolite/ degradant/ unknown structural elucidation

• Interpretation of elemental composition

• Mass Defect Filter

• Nitrogen Rule

• Ring Double Bond

• Interpretation of MS/MS data-Narrowing the site of Metabolic modification

• Utility of Hydrogen--Deuterium Exchange for structural Elucidation

• HRMS options for Drug-to-Antibody (DAR) ratio determination

• HRMS for biomarker discovery

 

Quantitative Analysis

• Key variables in HRMS quantitation:

– Operating resolution during acquisition

– Selection of m/z for quantitation: peak summing; multiply charged analytes; resolved isotopic envelopes

– Data processing peak widths

– Processing of centroid vs. profile data

• Data mining for metabolites, biomarkers and other non targeted and non-anticipated components

• ADC Payload Quantitation

 

Tutor

Dr. Ragu Ramanathan 

Bioanalytical Group Pfizer, Inc. Groton, CT, USA

Dr. Ragu Ramanathan is a Director of Global Small Molecule Bioanalysis in the Global Bioanalytical Laboratories at Pfizer Inc. (CT, USA). Ragu earned his BS degree in Chemistry from the University of Southern Mississippi (MS, USA) (1988) and a PhD degree in Analytical/Physical Chemistry from the University of Florida (FL, USA) (1994). Ragu’s postdoctoral research was performed at Washington University (MO, USA) (1995-1997). Through a research grant from the NIH, at Washington University Mass Spectrometry resources, Ragu expanded his training in mass spectrometry through application to breast cancer research and protein characterization.  From this training, Ragu’s interest ventured into application of mass spectrometry at several pharmaceutical research and contract research organizations, including Analytical Bio-Chemistry Laboratories (MO, USA), Schering-Plough Research Institute (now Merck; NJ, USA), Warner-Lambert (now Pfizer; MI, USA), Bristol-Myers Squibb (NJ, USA), QPS (DE, USA), and Pfizer, Inc. (CT, USA). Through collaborations and direct contributions, Ragu was fortunate to have published over 65 peer-reviewed research papers and over 12 book chapters in pharmaceutical bioanalysis, drug metabolism, metabolite identification, ion-molecule reactions, high resolution mass spectrometry and clinical biomarkers. 

Registration fee

200 Delegates

Registration fee

100 Students